CP504_Biological Process Engineering

Content	Time allocated (in clock hours)
Content	Lecture	Tutorial	Project	Assign.
Biological systems for the production of commercial goods and services: foods, drugs, chemicals, fuels, equipment, diagnostics, and waste treatment. Properties of microbial, plant and animal cells, and of enzymes used in bioprocess applications. Elementary aspects of molecular biology, biochemistry, and microbiology.	04 01 01			30
Enzyme kinetics and associated reactor design. Cellular kinetics and associated reactor design. Sterilization. Downstream processing. Biochemical and biological constraints on mass transfer, heat transfer, mixing and rheology. Control of bioreactors.	04 04 05 03 02	02 02 02
TOTAL (equivalent hours)	24	06		15

Note: Assign. stands for Assignment

Suggested Reference Texts

- Lee JM, 1992, Biochemical Engineering, New Jersey: Prentice-Hall

- Shuler ML and Kargi F, 2005, Bioprocess Engineering - Basic Concepts, Second Edition, New Delhi: Prentice-Hall of India

- Aiba S, Humphrey AE and Millis NF, 1973, Biochemical Engineering, Second Edition, New York & London: Academic Press

Assessment Scheme

Assessment method	Percentage marks
Continuous assessments	25
Assignments		25
Mid-semester examination	25
End-of-semester examination	50

Timeline for Lectures and Tutorials (modified on 15 Dec 2012)

Week	Cumulative hours	Date	Content
1	Lecture hr 01	09 Nov	Introduction to biological process engineering (ppt_Set_01 Enzyme kinetics and associated reactor design: Introduction to enzymes, enzyme catalyzed reactions and simple enzyme kinetics (ppt_Set_02, handout)
	Lecture hr 02		continued
2	Lecture hr 03	16 Nov	Enzyme kinetics and associated reactor design: Determination of the kinetic parameters of enzyme-induced reactions (ppt_Set_03)
	Lecture hr 04		Enzyme kinetics and associated reactor design: Immobilized enzymes (ppt to be uploaded when this section is taught on a later date)
3	Lecture hr 05	23 Nov	Enzyme kinetics and associated reactor design: Enzyme reactor design (ppt_Set_05)
	Lecture hr 06		Tutorial
4	Lecture hr 07	30 Nov	Tutorial
	Lecture hr 08		Overview of biological basics for engineers (ppt_Set_06, handout)
5	Lecture hr 09	07 Dec	Cellular kinetics and associated reactor design: Reactor design for cell growth (ppt_Set_07)
	Lecture hr 10		continued
6	Lecture hr 11	14 Dec	Cellular kinetics and associated reactor design: Modelling cell growth (ppt_Set_08)
	Lecture hr 12		continued
7	Lecture hr 13	21 Dec	Class is postponed to an extra hour in week 08 before midsem
	Lecture hr 14		Class is postponed to an extra hour in week 08 before midsem
		28 Dec	X’mas Break
			X’mas Break
8	Lecture hr 15	04 Jan	Mid semester Examination (Sample problems can be found at http://www.che.ncsu.edu/haughlab/bioche_probs.html)
	Lecture hr 16		Mid semester Examination continued
9	Lecture hr 17	11 Jan	Lecture cancelled owing to water supply disruption
	Lecture hr 18		Lecture cancelled owing to water supply disruption
10	Lecture hr 19	18 Jan	Sterilization (ppt_Set_09)
	Lecture hr 20		continued
11	Lecture hr 21	25 Jan	Holiday
	Lecture hr 22		Holiday
12	Lecture hr 23	01 Feb	Sterilization continued (ppt_Set_10)
	Lecture hr 24		continued
13	Lecture hr 25	08 Feb	Lecture cancelled
	Lecture hr 26		Lecture cancelled
14	Lecture hr 27	15 Feb	Downstream processing (ppt_Set_11; lecture notes on some sections handed over)
	Lecture hr 28		continued
15	Lecture hr 29	22 Feb	Biochemical and biological constraints on mass transfer, heat transfer, aeration, agitation, scale-up and rheology (ppt_Set_12; Asanka’s presentation)
	Lecture hr 30		continued

Past Examination Papers

Past mid-semester examination papers			Past end-of-semester examination papers
June 2005	July 2006	March 2009	Aug 2005	Sept 2006	May 2009
Nov 2011	Nov 2011 (makeup)		Jan 2012

Assignment related Information (updated in consultation with students)

Self Study 01: Get the rate equations for substrate and product inhibition in enzyme reactions.

Self Study 02: Estimate the kinetic constants of the Monod model (click here)

Self Study 03: The enzymatic isomerization of glucose to fructose (click here)

Self Study 04: Enzyme use in the industry (click here)

Literature Survey type assignments:

Recommended research papers for literature survey type assignment:

· Amenaghawon NA, Okieimen CO, Ogbeide SE (2012) Kinetic Modelling of Ethanol Inhibition during Alcohol Fermentation of Corn Stover using Saccharomyces Cerevisiae. International Journal of Engineering Research and Applications (IJERA), 2(4): 798-803. URL: http://www.ijera.com/pages/v2no4.html (pdf)

· Xavier JB, Picioreanu C, van Loosdrecht MCM (2005) A framework for multidimensional modelling of activity and structure of multispecies bioﬁlms. Environmental Microbiology, 7(8): 1085–1103 (pdf)

· Rios GM, Belleville MP, Paolucci D, Sanchez J (2004) Progress in enzymatic membrane reactors–a review. Journal of Membrane Science, 242: 189–196 (pdf)

· Gimbun J, Dayang Radiah AB, Chuah TG (2004) Bioreactor design via spreadsheet – a study on the MSG process. Journal of Food Engineering, 64:277-283 (pdf)

· García-González CA, Alnaief M, Smirnova I (2011) Polysaccharide-based aerogels—Promising biodegradable carriers for drug delivery systems. Carbohydrate Polymers, 86: 1425–1438 (pdf)

Simulation type assignments:

(15% bonus)

(15% bonus)

(40% bonus)

(50% bonus)

(50% bonus)

(75% bonus)

Literature for SimAssign 07

E number	Student name	Assignment presentation/ submission date (time)	Assignment type / topic
E/04/146	Karunathilake T.D.U.S.
E/07/081	Dorakumbura D.K.M.P.H.B.S.		Simulation Assignment 7
E/07/179	Karunathilake N.M.S.S.B.
E/08/011	Amarasekare K.L.L.R		Simulation Assignment 4
E/08/018	Ariyarathna R.G.R.N.K
e/08/032	Bandara E.A.P.C
e/08/113	Illukwtta I.A.C
E/08/124	Jayasinghe G.A.S.K
E/08/217	Nawarathna W.P.G.S		Simulation Assignment 6
E/08/274	Rathnayaka M.N.S
E/08/311	Senarathne R.A.R.C
E/08/333	Subhashini W.R.A.I
e/08/356	Thilakarathna H.T.C
E/08/394	Wijerathne W.D.C.C		Simulation Assignment 7


email: rshanthini@pdn.ac.lk	copyright © R. Shanthini	updated on Feb 28, 2013